Nassau County police say the latest incident happened on Seward Drive in Woodbury.
Multiple homes were hit with silver spray paint saying "MS13" and various profanities between 9 p.m. Friday and 6 a.m. Saturday, investigators said.
MS-13 is the El Salvador-based street gang blamed for more than 20 killings on Long Island in the past year and a half.
On Aug. 27, Nassau police said graffiti with "MS13," profanities, swastikas and other anti-Semitic messages was spray-painted overnight on Syosset High School.
It's not clear whether the same people may be involved in both episodes.
Detectives request anyone with information about the above crime to contact Nassau County Crime Stoppers at 1-800-244-TIPS. All callers will remain anonymous.
(The Associated Press contributed to this report.)