He was 3-for-5 and is two hits shy of 2,500 for his career. He is batting .571 (16-for-27) over his past six games, including five multihit efforts.
Pettitte (13-9) had not won since beating Boston on July 26, losing twice and taking two no-decisions in between. He allowed one run and five hits in seven innings, walked none and struck out four. He set down 10 straight at one stretch and threw 83 pitches, including 62 strikes.
Brian Bruney got the final six outs for the Yankees, who have won three of four after losing six of their previous seven.
Toronto rookie left-hander David Purcey (2-4) allowed five runs and seven hits in four innings. He walked two and struck out two.
Purcey was in immediate trouble, giving up consecutive singles to Johnny Damon, Jeter and Bobby Abreu to begin the game. Alex Rodriguez flied out to shallow right, but Jason Giambi hit a sacrifice fly and Xavier Nady followed with an RBI single.
New York added three runs in the fourth. Robinson Cano walked, went to third on Jose Molina's single and scored on Damon's fielder's choice before Jeter hit his two-run drive to right.
Alex Rios, whose 26-game hitting streak against the Yankees ended Tuesday, drove in Toronto's only run with an RBI single in the sixth.
Damon, who dropped two routine fly balls in Tuesday's 2-1 loss, shared a smile with left fielder Nady after catching Lyle Overbay's fly ball in the seventh, his first and only chance of the night.
Damon, who came in hitless in 11 at-bats, finished 2-for-4 with a walk.
Playing his second game since coming off the 15-day disabled list Tuesday, Yankees designated hitter Hideki Matsui went 1-for-4.
He hit a one-out double in the eighth, but was caught off the base when Cano lined out to first for an inning-ending double play.
