The show at Manhattan's Park Avenue Armory show reproduces the 15th century painting with a 40-minute sound-and-light show.
The "clone" painting is set within a full-scale replica of the 4,000-square-foot dining hall at Santa Maria Delle Grazie. The convent in Milan, Italy, houses the original work.
"Leonardo's Last Supper: A Vision by Peter Greenaway" opens Friday and ends Jan. 6.
The work by the Welsh-born filmmaker and multimedia artist fills the armory's cavernous drill hall.
The armory's president, Rebecca Robertson, calls Greenaway's installation "an incredible multimedia reverie."