The privately-operated bus collided with a car in the bus lane of the westbound helix just before 8 a.m.
The driver of the car was injured and transported to a nearby hospital.
Four people on the bus were taken to Palisades Medical Center with non life-threatening injuries.
The crash closed the express bus lane for the morning commute.
Only buses were allowed into the outbound tunnel, and they were running with 45 to 60 minute delays. Other buses were diverted to the Secaucus Junction Station.
Bus service to and from the George Washington Bridge Terminal was also subject to 20-minute delays.
Outbound cars were diverted to the Holland Tunnel and George Washington Bridge.
NJ Transit trains were also cross-honoring bus tickets and passes to and from New York Penn Station.