The Port Authority of New York and New Jersey will close the bridge between 9 p.m. and 5 a.m. on Monday, Tuesday and Wednesday as part of the $1.3 billion raising of the roadway.
Traffic will be diverted to the Goethals Bridge.
Also on Monday, the JFK Boulevard northbound on-ramp and southbound off-ramp in Bayonne will be closed for the duration of the project.
The bridge-raising project will raise the 82-year-old bridge's roadway by 64 feet in order to accommodate larger cargo ships anticipated to begin using the expanded Panama Canal by the end of 2015.