The crash happened at about 7:20 p.m. on airport property at Marine Terminal Road and Runway Drive, an access road.
Port Authority police say a 36-year-old man on a bicycle was struck by a vehicle. He was pronounced dead at Elmhurst Hospital.
The victim was identified as Steven Morales of Queens.
Morales was an employee of Swissport International Ltd, a Swiss aviation services company providing airport ground and cargo handling services.
It is not yet known what type of vehicle struck the man.
Runway Drive was closed in both directions between 94th Street and Marine Terminal Road for the investigation but later reopened.
* More Queens news
* Send us a news tip
* Download the abc7NY app for breaking news alerts