The call came in at about 9:40 a.m. for the two-alarm fire at a two-story brick home on 117th Street near Atlantic Avenue in Richmond Hill.
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Over 100 firefighters from 25 units responded to the blaze.
A 51-year-old woman and a 7-year-old girl, identified as Silvia Umana and Guadalupe Perez, were rushed to Jamaica Hospital and pronounced dead.
A 19-year-old male was hospitalized in critical condition and a 15-year-old male who jumped out a window to escape the flames had non-life threatening injuries.
Police announced Thursday that the 19-year-old, identified as Gilbert Diaz, also died from his injuries.
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Two firefighters suffered minor injuries. The fire was declared under control shortly before 11 a.m.
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