Firefighters responded to the 14-story building at 650 W. 42nd St. between 11th and 12th avenues just after 12:30 p.m.
The fire broke out on the seventh floor of the building and was extinguished.
In total, 78 firefighter and EMS personnel responded to the scene.
The two injured civilians suffered minor injuries and were transported to a hospital for evaluation.
The cause of the fire is under investigation.
ALSO READ | Exclusive: New York City rejects couple's request to pay for car totaled during NYPD pursuit
----------
* Get Eyewitness News Delivered
* More Manhattan news
* Send us a news tip
* Download the abc7NY app for breaking news alerts
* Follow us on YouTube