The incident happened at around 2:30am along Springfield Boulevard near 112th Avenue in Queens Village.
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The three injured firefighters had to be extricated following a collapse at the rear of the building.
Two have been hospitalized with injuries that are described as serious but non-life-threatening.
A barber shop owner says he fears that he may have lost his business.
Some of the other affected businesses included a restaurant and hair salon.
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Authorities have no word yet on the cause of the fire.
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