It happened on Townline Road and Terry Road at 6:43 a.m.
The car then crashed into a tree.
The three construction workers were contractors working for Suffolk County on a paving project.
One man was was taken to the hospital where he was pronounced dead, another was seriously injured, and the third suffered non-life-threatening injuries.
The driver was seen being placed into handcuffs and taken into police custody.
The crash is under investigation.
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