Firefighters battled flames at the home at 2925 Throop Avenue around 11:30 p.m. and got the fire under control after midnight.
First responders transported the victims to Jacobi Medical Center with critical injuries. A firefighter was also transported to a local area hospital for treatment of a non-life-threatening injury.
The FDNY says hazmat resources are evaluating an e-bike found at the scene. More than 100 fire and EMS personnel responded to the blaze.
Fire marshals are still investigating the cause of the fire.
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