The 85-year-old woman was discovered unconscious and unresponsive in the 26th Avenue home just after 5:10 a.m.
The fire started on the first floor of the two-and-a-half story home.
Seventy firefighters responded to the scene. The fire was under control by 6:37 a.m.
The woman was rushed to New York-Presbyterian Queens Hospital in critical condition where she was later pronounced dead.
Two firefighters also suffered minor injuries.
The cause of the fire is under investigation.
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