Firefighters responded to the 14-story building at 650 W. 42nd St. between 11th and 12th avenues just after 12:30 p.m.
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The fire broke out on the seventh floor of the building and was extinguished.
In total, 78 firefighter and EMS personnel responded to the scene.
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The two injured civilians suffered minor injuries and were transported to a hospital for evaluation.
The cause of the fire is under investigation.
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