After being shot, the victims' black Ford Edge crashed into a line of parked cars on Parkside Avenue in Prospect Park just before 3:30 a.m. Friday.
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A 42-year-old man was shot in the left arm, neck and left jaw and was pronounced dead at Maimonides Medical Center.
A 20-year-old man was shot in the back. He was in stable condition at Kings County Hospital.
A third man, a 22-year-old who was also sitting in the vehicle, was not shot.
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The two suspects on bicycles shot into the SUV and then fled eastbound on Parkside Avenue.
No arrest were immediately made and the motive is not known.
In a separate incident, a 22-year-old Brooklyn man was fatally shot by a gunman on Citi Bike Thursday night.
Storm Hughes was pronounced dead at Brookdale University Hospital, where his mother is an administrator.
The gunman, who fled the scene on a Citi Bike, was later taken into custody.
The shooting appears to be over marijuana. Hughes has no prior arrests.