It happened at the Carve Cafe in Midtown around 4:15 a.m.
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Authorities say a man and woman got into a dispute with another customer inside the 24-hour pizzeria.
When the employee went to break up the argument, he was stabbed with some type of object by the woman, police say.
The 61-year-old man was rushed to the hospital and is expected to survive.
The pair fled the scene after the stabbing, and authorities continue to search for them.
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Police describe the man as white, wearing a gray hooded shirt and carrying a backpack.
The woman is described as having a light complexion, wearing blue jeans, a dark waist-length jacket, and black and orange sneakers.
Anyone with information in regard to the identity of these individuals is asked to call the NYPD's Crime Stoppers Hotline at 1-800-577-TIPS (8477) or for Spanish, 1-888-57-PISTA (74782).
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