The victim, a man in his 20s, was stabbed in the stomach on Hylan Boulevard in the Old Town section just before 12:45 p.m.
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He was rushed to Staten Island University Hospital North, where he is listed in stable condition.
Authorities say a man and woman fled on foot but were quickly detained.
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Michael Lightfoot, 30, is charged with assault, misdemeanor assault, criminal possession of a weapon and menacing.
Christine Riley, 28, is facing charges of assault, criminal possession of a weapon and menacing.
Investigators are still determining a possible motive.
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