Police say a 25-year-old man and two women, ages 21 and 24, were slashed on Christopher Street around 2:30 a.m.
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They were all taken to Bellevue Hospital in stable condition.
At least one person was arrested.
The slashings ended a day and night of protests and partying on Stonewall riots anniversary.
The protest, dubbed the Queer Liberation March, started as a peaceful march from Foley Square into Washington Square Park, where it became violent just after 4 p.m.
At Washington Square Park, police say they caught 29-year-old Michael Dunn writing graffiti on an NYPD cruiser. The arrest sparked pushing between protesters and police that resulted in two additional arrests.
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Dunn, 20-year-old Millen Dang and 25-year-old Jacob Kruger were all charged with assaulting a police officer, resisting arrest, and obstructing governmental administration.
The protest then made its way to the Stonewall Inn, where thousands spent the night on Christopher Street
The Queer Liberation March was meant as a smaller alternative to the city's massive annual Pride march, which was canceled by the pandemic.
The protesting marked the Stonewall uprising, which started in the early morning hours of June 28, 1969, when police raided the Stonewall Inn, a well-known gay bar on Christopher Street. LGBTQ advocates fought back against the police crackdown, sparking days of running battles in the Village.
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