Police said a gunman opened fire on a northbound 4 train at around 12:50 a.m.
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It's unclear exactly where the shooting took place.
Shell casings were recovered from the Burnside Avenue station.
The suspects, described as a male and female, both Hispanic, in their 20s, are believed to have fled at the 183rd Street station.
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The train was held at the Fordham Road station as police investigated and northbound 4 trains were experiencing delays.
There were passengers on the train at the time, but there were no reports of any injuries.
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