American Flight 1680 was bound for LaGuardia Airport in New York City from Miami when the Boeing 737 was forced to land at Jacksonville International Airport in Florida around 6:30 p.m.
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The airline said the diversion was due to a "disruptive incident involving a customer."
The aircraft was cleaned after diverting and continued to LaGuardia without further incident.
According to TSA's website, pepper spray is not allowed in carry-on bags but is allowed in checked baggage.
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The Federal Aviation Administration (FAA) will investigate.
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