The 45-year-old, identified as William Alvarez, was fatally injured on a southbound D train at the 182-183 Street station just before 5:30 a.m.
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He was taken to St. Barnabas Hospital where he was pronounced dead.
NYPD officials say Alvarez was sitting on the train when two men and a woman boarded at the Fordham Road station.
One of those men, police say, sat next to the victim and words were exchanged.
The two started arguing and Alvarez was surrounded by the two additional passengers who were riding with the initial suspect.
He was fatally injured when the train reached the station.
"The whole incident was recorded on a camera that was on the subway car," MTA Chair and CEO Janno Lieber said.
Police say it appeared he had been either shot or stabbed and that the medical examiner will make the final determination about what kind of weapon was used.
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The three suspects seen in the surveillance images left the train at the 182-183 Streets station, leaving the victim to die of his wounds.
The motive remains "unknown. We do know that the victim wasn't the primary aggressor in this case," NYPD Assistant Chief Jerry O'Sullivan said.
Along with the entire incident captured on surveillance camera, "there were other riders on that car and there were other riders on the cars adjoining," NYPD Transit Chief Michael Kemper said. "We do have eyewitnesses and ear witnesses."
The station remained closed into the morning while authorities conducted their investigation.
Neighbors said they were shaken by the deadly incident.
"I know to avoid this station because it's typically a dangerous station, so I typically take the station ahead or behind and travel from there, it's terrible... the train is not safe," the neighbor named Cameron said.
No weapon was recovered and no arrests have been made.
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Alvarez's death comes on the heels of the transit system seeing an uptick with overall crime up by 22.6%
"We need a lot more safety, a lot more protection because this is unacceptable, I mean a stabbing, a shooting every other day in New York, it's not supposed to be like this," subway rider Carl Ducena said.
Just a week and a half ago, a man was killed and five others were injured in a shooting at the Mt. Eden station during rush hour.
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