Midfielders Jermaine Jones and Sacha Kljestan have left the U.S. training camp and will miss Friday's exhibition against Jamaica at Chattanooga, Tennessee.
The U.S. Soccer Federation said Monday that Jones is reporting to the LA Galaxy and Kljestan is awaiting the birth of his child.
Both started Sunday's 0-0 tie against Serbia in San Diego, the first game for the Americans since Bruce Arena replaced Jurgen Klinsmann and started his second stint as coach.
Twenty-one players remain on the roster for Friday's game. Europe-based players will miss the match but will return for World Cup qualifiers against Honduras on March 24 at San Jose, California, and at Panama four days later.
The U.S. is trying to overcome a 0-2 start in the final round of World Cup qualifying in North and Central America and the Caribbean.
The revised squad:
Goalkeepers: David Bingham (San Jose), Nick Rimando (Salt Lake), Luis Robles (New York Red Bulls).
Defenders: DaMarcus Beasley (no club), Steve Birnbaum (D.C. United), Brad Evans (Seattle), Greg Garza (Atlanta), Chad Marshall (Seattle), Jorge Villafana (Santos Laguna, Mexico), Walker Zimmerman (Dallas), Graham Zusi (Kansas City).
Midfielders: Alejandro Bedoya (Philadelphia), Michael Bradley (Toronto), Benny Feilhaber (Kansas City), Sebastian Lletget (LA), Dax McCarty (Chicago), Darlington Nagbe (Portland), Chris Pontius (Philadelphia).
Forwards: Juan Agudelo (New England), Jozy Altidore (Toronto), Jordan Morris (Seattle).