Sheriff's deputies blocked entry to Mac's Public House following a judge's ruling on Thursday.
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She found that Mac's was serving alcohol without a license.
The state suspended the license last year because the owners were not adhering to Governor Cuomo's shutdown orders for bars.
Attorneys for the pub said the city dismissed more than 25 criminal summonses for the establishment in December after it stayed open for indoor dining and past curfew despite being in an orange zone amid a surge in coronavirus cases.
General manager Daniel Presti was arrested twice in the span of a week during the Sheriff's Department actions, sparking a massive protest outside.
He and owner Keith McAlarney said the rulings were a victory for small business owners.
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