The latest incident happened Friday afternoon.
Eyewitness News reporter CeFaan Kim says he's hearing a small group of protesters may have been there at the time.
Vandals strike 'Black Lives Matter' mural outside Trump Tower again
Police arrested Juliet Germanotta, 39, Luis Martinez, 44, and D'Anna Morgan, 25, and charged them with criminal mischief. They were released with a desk appearance ticket.
In addition, a 64-year-old woman was arrested and given a criminal court summons for illegal posting of flyers.
A few hours later, the mural was being repaired by DOT crews.
The first incident happened back on Monday. Police were still looking for one person in that case who walked up to mural and dumped red paint over the letter V in "Lives," which splattered across the mural as cars drove over it.
Video obtained by Eyewitness News shows the vandal in action:
Video shows Black Lives Matter mural vandalized
"I saw somebody out of the corner of my eye putting down a bag... and I was thinking 'oh he's gonna get in my shot,'" Nikki Ross, who recorded the video, told Eyewitness News. "And when I got to about the clock, he took out the paint and splattered it across the mural."
Ross said the last few months have awakened her to her privilege and it's her responsibility as a white woman to call out injustice.
"To be vocal, to tell your friends what the movement is about and explain to your friends how Black Lives Matter," Ross said. "I'm glad that he left the paint can there and wasn't wearing gloves."
Mayor Bill de Blasio said later Monday evening that the mural had already been fixed.
"The #BlackLivesMatter movement is more than words, and it can't be undone," he said on Twitter.
Vandal defaces BLM mural outside Trump Tower
The suspect was described as a white male wearing a black hat and a black T-shirt who fled westbound on West 56th Street.
It is unclear whether he had a political motivation or if he was just looking for attention. But cops say it's still an act of criminal mischief which is a misdemeanor.
The NYPD is now reviewing surveillance videos, and anyone with information is asked to contact the police.
Related: 'Black Lives Matter' painted in front of Trump Tower on 5th Avenue
'Black Lives Matter' mural painted in front of Trump Tower
----------
* Get Eyewitness News Delivered
* More New York City news
* Send us a news tip
* Download the abc7NY app for breaking news alerts
* Follow us on YouTube