Precious Arzuaga and Jennifer Quijano were convicted of first-degree murder.
The U.S. Department of Education is investigating complaints that some districts are allowing students to compete in sports based on gender identity, rather than biological sex.
Lower courts ruled for the transgender athletes in Idaho and West Virginia who challenged the state bans, but the conservative-dominated Supreme Court gave no indication after more than three hours of arguments that it would follow suit.
The Supreme Court will decide whether states can ban transgender girls from participating in girls and women's sports under Title IX and the Constitution.
He said the four day athletic event would include "the greatest high school athletes, one young man and one young woman, competing from each state and territory."
The U.S. Department of Health and Human Services unveiled a series of regulatory actions designed to block access to gender-affirming care for minors, building on broader Trump administration restrictions on transgender Americans.