The preppy teen clothing seller says it closed a Hollister store on Thursday in the SoHo neighborhood of New York. That location is expected to open at 10 a.m. on Saturday.
It closed a second location, an Abercrombie & Fitch store in the South Street Seaport, on Friday for the same reason.
Abercrombie's flagship New York store on Fifth Avenue has been tested and is not affected by bed bugs, the New Albany, Ohio, company says.
Abercrombie says it asked New York City's Mayor's office how it should deal with the problem.
Shares fell 64 cents, or 2 percent, to $31.27 during afternoon trading.