The Great Neck Village board of trustees refused this week to lift a ban on the birds.
Now, Alfred Basal must find a new home for Fluffy, Cutie and four other chickens.
The birds are considered a nuisance under village ordinance.
Basal bought the chickens about a year ago and has been keeping them in a backyard coop. He was issued a notice of violation in February. He appealed, arguing that the chickens were educational because they produce about six eggs a day.
Newsday reports that some neighbors expressed concern about salmonella, avian influenza and noise from the birds' clucking.
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