The mayor said in a statement Wednesday that he had decided to divorce from his wife "after exhausting every other recourse."
Adam Bradley requested privacy, but the divorce is likely to come up in his criminal case.
Bradley is accused of throwing hot tea at wife Fumiko Bradley and slamming a door on her hand. He is also charged with harassment and witness tampering. The 48-year-old mayor has pleaded not guilty and resisted calls to resign.
He was arrested in February, two months into his term.
A call to Fumiko Bradley's lawyer, Neil Comer, was not immediately returned.
